We propose a comprehensive study of the generalized eight-parameter Mittag-Leffler (8P-ML) function and its operational properties under various fractional calculus operators. Explicit results are derived for its integer and fractional-order derivatives, integrals, Laplace and Fourier transforms, and convolution identities. These findings extend classical Mittag- Leffler (M-L) theory and provide foundational tools for applications in anomalous diffusion, viscoelastic modeling, and fractional dynamic systems. The results are presented in a unified framework, offering deeper insights into the structural behavior of this highly generalized function.