In the framework of q*-rung orthopair neutrosophic topological space(q*-RONTS), this study focuses on establishing and analyzing separation axioms, such as N*qn T0, N*qn  T1 and N*qnT2. The paper explores the manifestation of these axioms within the broader framework of q*-rung orthopair neutrosophic (q*-RON) theory, taking into account the complex role of degrees of truth, falsehood, and indeterminacy. Additionally demonstrate how the unique structure of q*-RONTS influences the separation of sets and points using in-depth definitions, theorems, and examples. The analysis of the impact of separation qualities in q*-rung orthopair neutrosophic topoligical subspaces and how these properties are maintained or changed under continuous mappings is also included in this paper.