Purpose: The scientific impact of every scientific publication is widely measured through bibliometric analysis. In this article we have conducted evidence-based bibliometric analysis of articles on nanotechnology. Literature on nanotechnology applications that has been published in journals across all fields of science and technology. The data is retrived from Medline database via Pubmed., Total 267 publications were retrieved and analyzed, The New England Journal of Medicine achieved the highest citation, “Corcoy Rosa” is the most productive author. Most of the publications are classified as Randomized controlled trials, despite this, there hasn’t been a lot of in-depth research on the publication trends of nanotechnology in journals of the health sciences. Objective: The objective of this study was to evaluate the publication trends in the field of Nanotechnology with special emphasis on health sciences.  Methods: The data was acquired from the MEDLINE database via PubMed on January 4, 2021, covering the years 2001 through 2020. The articles were sorted according to the Publication Type, Number of Publications (Pub), Relative Citation Ratio (RCR) and Total Citations (TC) influence. The tables are created in excel spread sheet which is download from “iCite” tool. Highly cited journals and author tables are created usning “VOS Viewer” visualization bibliometric tool. Findings: A total number of 267 records were retrieved, and a systematic review was carried out over 20 years between 2001 and 2020. The total number of RCR and citations are 545.44 and 5307 respectively. The New England Journal of Medicine (NEJM) received more citation which is n=214. “Corcoy Rosa” was the most productive author during the study period, with n=9 publications with 608 citations aggregated. Most of the publications are classified as Randomized controlled trials, which account for 37% of all publications retrieved. The year 2019 has the most publications, with a total of n=29. European countries have leading publications and authors in this field of research; however, the rest of the world needs to apply more concentration to improve the development of this field. Limitations: The study was limited to twenty years period from 2001 to 2020, and the data was obtained from single database PubMed.